Veterinary Immunohistochemistry (IHC) Database

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  • Immunohistochemistry
    • Infectious Agents >
      • Bacteria
      • Fungi
      • Protozoal and Prion Diseases
      • Viruses
    • Cellular Markers
  • PCR
    • Bacteria
    • Genetic >
      • Canine
      • Feline
      • Other
    • Viruses
  • Toxicology
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Toxicology

Toxin/substance

Panel components/

Notes

Sample needed

Laboratory

25-OH-D3

Vitamin D3 poisoning (not Dovonex)

 

Half a Kidney

DCPAH

Acetylcholinesterase

Chill

EDTA, heparinized serum or plasma;

entire hemisphere of brain

WSU

Acid extracted drugs

Acetaminophen, barbiturates, ibuprofen, flunixin (see link for full list)

4 mL heparinized plasma, 10 mL

heparinized whole blood, 50 mL urine

COR

Aflatoxin

Chill

250 g of feed

WSU

500 g of feed

TVMDL

20 g liver, 100 mL milk

ISU

Contact lab

PUR

Aflatoxin B1

 

>100 g feed (NDSU); 500 g feed (UCD)

NDSU; UCD

Aflatoxin M1

 

400 mL milk

UCD

Aflatoxin quantitative (ELISA)

 

110 g of feed

COR

Aflatoxin rapid screen

 

> 15 g feed

COR

Aldicarb

 

20 grams of stomach contents and/or 10 grams of bait

TVMDL

Alkaloid screen

Anabasine, atropine, coniine, deltaline, nicotine, scopolamine, sparteine, taxus

50 g GI contents, 20 mL urine, 25 g plant

 

UCD

Strychnine, brucine, caffeine, theobromine, theophylline, nicotine, atropine, cocaine, morphine, codeine, ephedrine, reserpine, quinine, and solanidine

Must contact lab before submission;

5 g liver, 10-20 ml heparinized whole blood,

50 ml urine, 100 g other materials 

COR

Amanitin

 

2mL bile, 5mL urine, 25 g GI content, 10 g mushroom.

 

UCD

Ammonia

 

EDTA or heparinized serum or ocular fluid

WSU

500 g frozen rumen content

TVMDL

Anabolic steroids

boldenone, testosterone, and stanozolol

50 ml urine, 10 ml heparinized whole blood, 4 ml plasma 

COR

Anatoxin-a

Cyanobacterial toxin

50 g GI contents, 50 mL water

UCD

Anesthetic screen

acepromazine, barbiturates, ketamine, morphine, xylazine, tiletamine/zolazepam

50 ml urine, 10-20 ml heparinized whole blood, 5 g liver 

COR

Anticoagulant screen

Warfarin, Bromadiolone, Brodifacoum, Diphacinone, Chlorophacinone, and Difethialone

2.0 mL EDTA blood, 2.0 mL serum, 10 g fresh liver, 10 g bait

TVMDL

Brodifacoum, bromadiolone, chlorophacinone, coumachlor, difethialone, diphacinone, and warfarin.

20 g liver, 5 mL serum, 5 mL blood (RTT), 20 g bait.

 

UCD

Brodifacoum, bromadiolone, chlorophacinone, coumachlor, coumafuryl, diphacinone, difethialone, pindone, valone, warfarin, difenacoum

20 g liver, bait or stomach contents;

5 mL blood (EDTA or heparin) or urine

WSU

Brodifacoum, bromadiolone, chlorophacinone, dicoumarol, difethialone, diphacinone, pindone, valone and warfarin.

Liver, whole blood, other

DCPAH

Brodifacoum, bromadiolone, chlorophacinone, diphacinone, pindone, warfarin

10 g liver, GI contents, 100 g bait (batched)

GOC

Unspecified

Contact lab

PUR

Anticoagulant screen-liquid

Brodifacoum, bromadialone, chlorophacinone, dicoumarol, difethialone, difenacoum, diphacinone, flocoumafen, warfarin

10-20 ml whole blood; 50 ml urine 

COR

Anticoagulant screen-solid

50g liver; 50g stomach contents; 200g feed

Arsenic

Chill

5 mL whole blood, urine, water, 2 g tissue or 100 g feed

COR

Blood (EDTA or heparin), urine; GI contents, source, liver or kidney cortex

WSU

Contact lab

PUR; CO

water

DCPAH

10 g liver or 10 g kidney, 500g rumen/stomach content, feed, or dirst/soil, 3 mL EDTA whole blood, 5 mL water; if chronic, 10 mL urine

TVMDL

Avicides

Avitrol and starlicide.

DCPAH, UCD- 20 g GI contents, 20 g source material; ISU- 50 g stomach contents, 50 g liver, 50 g bait; TVMDL-10 g stomach, crop, or rumen contents, 10 g bait

DCPAH; ISU; TVMDL; UCD

Basic extracted drugs

Acepromazine, Amphetamine, Apomorphine, Atropine, Benzocaine, Brompheniramine (see link for full list)

4 mL heparinized plasma,

10 mL heparinized whole blood, 50 mL urine

COR

Beetle identification

 

Blister beetle suspect

TVMDL

Β-lactam screen

Amoxicillin, ampicillin, ceftiofur, cephapirin, cloxacillin,

50 mL milk; Freeze and ship on dry ice.

UCD;

Blue green algae (Cyanobacteria) ID

 

Submit at least 500 ml. Keep sample cold.

KSU; NDSU

Bone ash

 

2.0 inch section of mid femur or humerus bone, minimum 10 g

TVMDL

Bone density

 

Contact lab

PUR

Bone screen

Unspecified

Contact lab

PUR

Bromethalin

 

10 g fat, 20 g brain, 20 g liver, 10 g bait

 

UCD;

Bromide

 

1-2 mL serum

COR; CO; DCPAH; TVMDL; UCD

Bronchodilators

Clenbuterol, Albuterol, Turbutaline

1) 10 mL heparinized whole blood; 2) 25 - 50 mL urine 

COR

Cadmium

 

1) 2g tissue; 2) 100g feed 

COR

Blood (EDTA/heparin) or urine; Liver, kidney (cortex)

WSU

5 g liver or kidney; 2 mL whole blood

CO

10 g liver, 10 g kidney

TVMDL

Caffeine

 

10 mL stomach contents, urine, serum

WSU

Calcium

 

Contact lab

PUR

50 g feed; 1 cm3 bone, 1 mL ocular fluid

CO

Serum, plasma, ocular fluid/intact eye

WSU

Calculi identification

 

Clean, dry uroliths

CO; TVMDL

Cantharidin

Blister beetle toxin

1) 20 mL to 1 L urine; 2) 500 g stomach contents, vomitus; 3) 10-20 mL serum

COR; TVMDL

Carbamate insecticide screen

Cholinestrase should be run on blood or brain

100 g frozen GI contents, 100 g frozen source material, 

100 g frozen feed, 50 g frozen liver, 10 mL blood.

 

UCD;

 

Contact lab

GOC

Chill/freeze

10 g Liver, kidney, GI contents, vomitus, lavage washings

WSU

Chloride

 

2 mL serum, 50 mL water

UCD

500g feed or rumen content, 50 mL water

TVMDL

Chlorinated compounds

beta HCH, DDT, DDE, and PCB

Dried blood spot on filter paper card

DCPAH

2,4-D, dicamba, bromoxynil, MCPA

10 mL serum or urine

WSU

Cholinesterase

 

Complete right or left half of brain, 

0.5-1 mL EDTA whole blood.

 

UCD

Brain, retina, whole blood

DCPAH

Contact lab

PUR

5 g brain, 1 mL serum or whole blood

CO

Entire brain, 5cc EDTA whole blood (ruminants, Avian sp.), 2cc serum (monogastrics)

TVMDL

Chromium

 

Liver or kidney

WSU

Cobalt

 

1 mL blood (EDTA or heparin), liver, kidney

WSU

5 g liver, 50 g feed, 2 mL serum/whole blood

CO

Copper

 

1 gram of tissue and/or 1 ml of serum

NDSU

Contact lab

PUR

5 g liver, 50 g feed, 2 mL serum/whole blood

CO

500g feed, 10 g liver, 10 g kidney; 2 mL serum or 10 mL urine

TVMDL

Convulsant panel

strychnine, tremorgenic mycotoxins (roquefortine, penitrem A) and bromethalin

Bait, stomach contents

DCPAH

strychnine, penitrem A, roquefortine

25 g GI contents, suspect material

GOC

Corticosteroid screen

Dexamethasone, Betamethasone, Methyl Prednisolone, Prednisolone, Prednisone, Flumethasone, Triamcinolone, and Triamcinolone Acetonide

20 mL heparin whole blood;

2) or transfer 5 mL heparin plasma into a red top tube; 3) 50 mL urine 

COR

Cyanide

Freeze sample promptly; Cannot analyze in plasma or serum

Stomach content, whole blood, liver, muscle, plants, bait and/or

source materials

 

DCPAH

Contact lab: 1) 10 mL EDTA whole blood;

2) 10 mL heparinized whole blood;

3) 50g stomach contents;

4) 100g forage, silage, or other material 

COR

Contact lab

PUR

50 g feed, 5 g lung, 5 mL whole blood

CO

8-15 stalks of fresh forage, hay core sample, frozen rumen contents, tied off stomach (small animal)

TVMDL

Cyanide (distillation)

 

Frozen 25 g muscle, 25 g liver, 10 mL blood, 100 g feed, 500 mL water

 

UCD;

Cyanide (qualitative screen)

 

UCD-Frozen 50 g plant material, 50 g GI content; WSU- 100 g feed

 

UCD; WSU

Cylindrospermopsin

Cyanobacterial toxin

500 mL water.

UCD;

Diacetoxyscirpenol

Fusarium spp. toxin

Contact lab

PUR

Dicumarol

 

20 g feed (UCD); 50 -100 grams forage (NDSU)

NDSU; UCD;

Domoic acid

 

5 mL urine, 5 mL serum.

 

UCD;

Drugs of abuse screen

Amphetamine, cocaine, benzoylecgonine, norcocaine, ephedrine, lysergic acid diethylamide (LSD), methamphetamine, MDMA, nicotine, phentermine

10 mL urine, 10 mL serum, 10 g liver, 10 g kidney

 

UCD;

Ethylene glycol

 

bait, stomach contents, plasma, liver and urine

 

DCPAH

Contact lab

PUR

1) 10 mL heparin whole blood; 2) 10 mL EDTA whole blood;

 3) urine; 4) food materials; 5) stomach contents 

COR

20 g stomach content, vomitus or bait

TVMDL

Ethylene glycol/ propylene glycol

Chill/freeze

5 mL serum, 10 mL urine, 50 g source material

WSU

Fat percent

Starvation

DCPAH, UCD- 50 g feed (UCD) or tissue (bone marrow);

PUR- bone marrow fat

UCD; DCPAH; PUR

Fluoride

 

Tissues, Bone, Horn, Hoof

UCD;

50 mL water

WSU

Fluphenazine

Anti-psychotic medication

10 mL heparine whole blood; or

2)   5 mL heparine plasma transferred to red top tube 

COR

Fumonisin

Mycotoxin

200 g feed

UCD;

100g feed, corn and corn feed, rice and rice containing feed 

COR

Contact lab

PUR

500 g feed

TVMDL

Gallotannins (Oak)

Urine analyzed for pyrogallol. Plant/GI content analyzed for gallic acid.

50 g GI contents, 5 mL urine, 50 g plant material.

 

UCD;

GC/MS Screen

Library of 100,000+ chemical/toxins

20 mL fluid, 50 g tissue, 50 g feed, 50 g source material.

 

UCD

Liver, stomach content

DCPAH

25 g tissue/other; contact lab

GOC

Glomerular filtration rate (iohexol clearance)

 

Live dog or cat serum

DCPAH

Glutathione peroxidase

Must be run within 7d of collection

Blood (EDTA, heparin)

WSU

Glycerol

 

5 g tissue or 5 g feed.

 

UCD;

Glycol acids

 

5 g tissue or 5 g feed.

UCD

1 mL serum or urine

TVMDL

Glycols

Ethylene glycol or propylene glycol

10 g GI contents, 10 mL urine, whole kidney, 

10 mL separated serum, 50 g source material.

UCD;

Gossypol

 

200 g feed or 5 mL serum/plasma

UCD;

Grayanotoxins I and III

Indicators of exposure to: rhododendron, azalea, mountain laurel and Japanese pieris

50 g GI contents, 10 g plant or feed material or 50 g feces.

 

UCD;

Heavy metal screen

Arsenic, cadmium, copper, iron, mercury, manganese, molybdenum, lead, zinc

10 g liver, 10 g kidney, 50 g source material, 

50 g feed, 50 g feces, 100 mL water

 

UCD;

Arsenic, Lead, Cadmium, Mercury, Thallium

1) liver; 2) kidney; 3) powders, solids; 4) paint chips 

COR

arsenic, cadmium, copper, iron, manganese, molybdenum, lead, zinc

1 g liver and kidney

WSU

arsenic, barium, cadmium, chromium, cobalt, copper, lead, manganese, molybdenum, nickel, selenium, vanadium, zinc

1 g liver, kidney

WSU

Antimony, Arsenic, Beryllium, Boron, Cadmium, Cobalt, Chromium, Copper, Iron, Lead, Mercury, Magnesium, Manganese, Molybdenum, Nickel, Selenium, Tin, Thallium, Zinc

10 g liver, kidney (cortex preferred), GI contents, 100 g feed 

GOC

Unspecified

Feed, fluid, premix, soil/sludge, water, tissue

DCPAH

Heavy metal/trace mineral combo

Antimony, Aluminum, Arsenic, Cadmium, Chromium, Cobalt, Copper, Iron, Lead, Manganese, Molybdenum, Nickel, Selenium, Thallium, Vanadium, and Zinc

Submit at least 1 gram of tissue

NDSU

Herbicide screen

detects a variety of herbicides

30 g stomach/rumen contents, 10 g bait (source material), 40 g of forage or hay, 20 g liver, 10 g crop contents

TVMDL

Insecticide screen

detects a variety of insecticides

30 g stomach/rumen contents, 10 g bait (source material), 40 g of forage or hay, 20 g liver, 10 g crop contents

TVMDL

Ionophore screen

Monensin, salinomycin, narasin, and lasalocid

50 g feed, 50 g GI contents; WSU also 50 g of bile; TVMDL- 500g feed or rumen/stomach content

 

UCD; DCPAH; TVMDL; WSU

Monensin, Lasalocide, Salinomycin

Feed

COR

monensin, narasin, salinomycin

100 g feed; does not include lasalocid

GOC

Iron

 

Contact lab

PUR; WSU

10 g liver, 500 g feed, 20 mL water

CO; TVMDL

Juglone

Black walnut toxicity

Contact lab

PUR

LCMS

 

20 mL fluid; 50 g 

tissue; 50 g feed; 50 g 

source material

 

UCD

Lead

Chill

1 mL whole (heparin/EDTA) blood, 10 g liver, 10 g kidney, 10 mL water, 25 g bone, 50 g source material.

 

COR; UCD; NDSU

0.050-0.5 mL blood (EDTA/heparin); liver, kidney (cortex)

WSU

Contact lab

PUR

5 g liver or kidney

CO

1 mL EDTA or Heparinized whole blood; 10 mL water; 10 g liver (monogastric), 10 g kidney (ruminant), or 10 g material (i.e. paint chips, feed, soil)

TVMDL

Macrolide endectocide screen

Ivermectin, moxidectin, selamectin and abamectin

Serum (4mL), brain, source material.

           

UCD

Magnesium

 

Contact lab

PUR

5 g liver, 50 g feed, 1 mL ocular fluid

CO

Serum, plasma, ocular fluid/whole eye, CSF

WSU

Manganese

 

1 mL blood (EDTA/heparin); liver, kidney

WSU

2 mL plasma or serum

CO

Mass spectroscopy screen

Special request

Contact before submission; 1) 10 mL heparin whole blood;

2) 20 - 50 mL urine; 3) tissue; 4) plant materials;

 5) stomach contents

COR

Melamine

 

Feed 50 g, Kidney 10 g, Urine 20mL, serum

UCD; DCPAH

5 g feed

WSU

Mercury

Need whole blood. Do not use heparin separator tubes

Whole blood

DCPAH

1 mL blood (EDTA/heparin); liver, kidney (cortex), GI contents

WSU

5 g liver, kidney, muscle, stomach contents, 5 mL urine, 2 mL whole blood; No serum, plasma or liver biopsies will be accepted

CO

1) 2g tissue; 2) 100g feed; 3) 2 mL heparin whole blood 

COR;

Metaldehyde

Freeze

50 g stomach content, 50 g source material,

 5 mL urine, 2 mL separated serum.

UCD;

10 g stomach contents, source

TVMDL; WSU

Metal screen

Arsenic, barium, beryllium, cadmium, cobalt, chromium, copper, iron, mercury, manganese, molybdenum, nickel, lead, thallium, vanadium

10 g liver, 10 g kidney, 50 g source material, 

50 g feed, 50 g feces, 100 mL water

 

UCD;

Methyl mercury

 

10 mL blood, 10 g liver, 10 g kidney, 10 g source material.

 

UCD;

Methylxanthines

Theobromine, theophylline and/or caffeine

Stomach contents, serum

PUR; DCPAH

Microcystins

 

50g GI contents, water 50mL.

UCD;

Minerals, toxic

Heavy metals

Feed, fluid, premix, soil/sludge, water, tissue

DCPAH

Mineral panel

Unspecified

COR: 1) 2g tissue; 2) 100g feed.

Do not submit serum or plasma;

NE- serum, water, feed, tissue (contact lab)

COR; NE

Molybdenum

Chill

1 mL plasma (EDTA/heparin); liver, kidney, GI contents

WSU

 

 

5 g liver, 50 g feed, 2 mL serum/whole blood. No liver biopsies accepted.

CO

Monensin/ Rumensin

 

100 g feed

GOC

Mushroom ID

 

Mushroom; Don’t put in plastic bag!

Ship wrapped in dry paper towel in paper bag

DCPAH; WSU

Mycotoxin LC/MS/MS Screen

 

Aflatoxin B1, B2, G1 and G2, T-2 toxin, Ochratoxin A and Sterigmatocystin at 20 ppb (0.02 ppm).

Fumonisin B1, B2, HT-2 toxin, and DON (vomitoxin) at 200 ppb (0.2 ppm).

Zearalenone at 50 ppb (0.05 ppm).

 

Submit at least 100 grams of sample

(quart size bag of grain, gallon size bag of silage, forage, hay).

NDSU

Aflatoxin B1, B2, G1, G2, Fumonisin B1, B2, B3, Nivalenol, Ochratoxin, T2, Vomitoxin, Zearalenone, Zearalenol

2 pounds of feed

ISU

Mycotoxin screen

Aflatrem,  Penitrem A, Ergotamine, Cyclopiazonic acid, Roquefortine

200 g feed; 50 g stomach/rumen contents

ISU

Aflatoxins, zearalenone, vomitoxin, DAS and T-2

Unspecified

Aflatoxin, deoxynivalenol, zearalenone

Aflatoxin, vomitoxin, ochratoxin, zearalenone

Feed

DCPAH

Contact lab

NE

Contact lab

PUR

500 g feed

WSU

Aflatoxins (B1, B2, G1, G2), Ochratoxin A, Zearalenone, T-2 Toxin, DAS, DON and Sterigmatocystin

100 g feed

COR

Nitrates

 

1.0 cc ocular fluid or entire eye ball, 1 cc urine, 1 cc serum, or 20 mL water; 8-15 stalks of fresh forage, or 1 jar filled 3/4 filled with a core hay sample

CO; TVMDL

Organochlorine insecticide screen

 

Frozen 50 g liver, fat or brain, 100 g source material, 

100 mL milk or GI content, 10 mL blood (not frozen)

UCD

1) 10 - 20 mL heparin whole blood; 2) 50 mL urine;

3) 50g stomach or rumen contents; 4) 50g liver; 5) 200g other materials 

COR

10 g fat, liver, kidney, brain; 50 g GI contents or source

WSU

contact

GOC

Organophosphorous insecticide screen

 

100 g GI content, 100 g source material or feed, 100 g feed, 50 g liver, 10 mL blood.

UCD

10 g liver, kidney; 50 g GI contents or source

WSU

1) 10 - 20 mL heparin whole blood; 2) 50 mL urine;

3) 50g stomach or rumen contents; 4) 50g liver; 5) 200g other materials 

COR

contact

GOC

Oxalate

 

1 plant, 50 g forage material, 50 g kidney.

UCD

Paraquat

Herbicide

100 g source/bait, 100 g lung, 50 g GI contents, 50 mL urine, 10 mL sera.

UCD

PCB screen

 

50 g liver, 50 g fat, 50 g brain, 100 g GI contents, 10 mL blood,

 100 g source material, 100 mL milk

UCD

Penitrem/ roquefortine

 

50 g source material, 5 mL serum, 5 mL urine, 5 g GI contents

UCD;

Perchlorate

 

Frozen 40 mL milk, 40 mL water, 10 g feed.

UCD;

Pesticide screen

Type not specified

COR- 1) 20 heparin whole blood; 2) 50 mL urine; 3) 500 mL water;

50g liver; 5) stomach contents; 6) 100g feed; 7) plant material;

NDSU- Minimum of 5 grams of stomach/rumen contents

COR; NDSU

Petroleum hydrocarbons

 

10 g liver, 50 g source material, 50 g feed.

UCD;

500 g GI content, 100 mL water, 100 g source material

TVMDL

Phosphate

 

2 mL serum, 50 mL water.

UCD;

Phosphine

Toxic gas; test for aluminum, magnesium and zinc phosphide rodenticides

500 g frozen stomach content, bait

DCPAH; TVMDL

Phosphorous

 

Serum, plasma, liver, kidney

WSU

50 g feed; 1 cm3 bone, 1 mL ocular fluid. No liver biopsies.

CO

Contact lab

PUR

Phytoestrogen screen

Daidzein, Genistein, Formononetin, Biochanin A, Coumestrol

Minimum of 100 grams of forage/feed.

NDSU

Plant identification

 

Don’t put in plastic bag! Wrap in paper towel and put in paper bag.

Intact plants from hay or pasture. Chill GI contents.

DCPAH; NDSU; TVMDL; UCD; WSU

Polychlorinated biphenyls (PCBs)

 

Fat and brain (necropsy) preferred but other tissue accepted, Serum (live)

DCPAH

Potassium

 

Liver or kidney

WSU

 

 

1 mL urine or ocular fluid; 50 g feed

CO

Praziquantel

 

50 mL water

UCD;

Pyrethrin screen

Allethrin, bifenthrin, cyfluthrin, cypermethrin, fenvalerate, permethrin, pyrethrins

100 g GI contents, 100 g source material, 100 g feed, 50 g liver, 

10 mL serum, 10 mL blood.

UCD;

Pyrethroid screen

Deltamethrin, Pyrethrum, Cis-permethrin, Permethrin, Cypermethrin, and α-cypermethrin

Fresh brain

TVMDL

Pyrrolizidine alkaloids

Groundsel, fiddleneck

GI contents, plant or source

UCD;

Ractopamine

Feed additive

Contact lab

CO

Reserpine

Equine sedation drug

10 mL heparin whole blood;

transfer 5 mL heparin plasma to a red top tube 

COR

Residues

Antimicrobial, growth promoters

Feed or milk

CO

Salt screen

Phosphorous, sulfur, magnesium, sodium, calcium, and potassium

10 g brain, eye (vitreous), 100 mL, water, 50 g source material, 

1 mL CSF, 50 g feed

UCD;

10 g liver, kidney, brain, 100 g feed

GOC

Saxitoxin

Shellfish toxin

500 mL water

UCD;

Selenium

 

5 mL whole blood, 10 g liver, 50 g GI contents, 50 g feed, 50 mL water

UCD; CO

L mL blood (EDTA or heparin); 1 g liver or kidney; 1 g hair

WSU

1) 2g tissue; 2) 100g feed; 3) 5 mL heparin whole blood;

4) 5 mL EDTA whole blood 

COR

Contact lab

PUR

2 cc EDTA whole blood, 2 cc serum, 10 g liver

TVMDL

Selenium/ Vitamin E screen

 

Contact lab

PUR

Single element analysis

Any element of interest

Serum, other tissue/substance

DCPAH

Sodium

 

Serum, plasma, CSF, brain

CO; WSU

Contact lab

PUR

Brain hemisphere

TVMDL

Sodium fluoroacetate

 

50 g feed, 50 g GI content (UCD); > 10 g (NDSU)

UCD; NDSU

Strophanthidin

Adonis exposure

10 g GI content, 10 g plant material.

UCD;

Strychnine

 

20 g GI contents, 10 mL urine, 20 g bait, 3 mL separated serum, 25 g liver.

DCPAH; UCD;

10 g stomach content/source; 2 mL serum; 10 mL urine; 25 g liver

WSU

1) 10 - 20 mL heparin whole blood; 2) 10 - 20 mL urine;

3) 50g liver; 4) 50g stomach contents or vomitus

COR

> 10-20 g stomach content

NDSU

20 g GI content or vomitus, 10 g bait, 20 cc of urine

CO; TVMDL

Sugar alcohols

 

5 g tissue or 5 g feed

UCD;

Sulfate

 

2 mL serum, 50 mL water

UCD;

Sulfites

Only validated for chicken jerky

2 g

UCD;

Sulfur

 

1) 2g tissue; 2) 100g feed 

COR

Contact lab

PUR

T-2 toxin

Fusarium spp. toxin

Contact lab

PUR

Thallium

 

5 g liver, kidney or 5 mL urine

CO

Theobromine

Chill/freeze

20 mL stomach contents, urine

WSU

Toxic element panel

Antimony, Arsenic, Beryllium, Cadmium, Chromium, Lead, Mercury, Nickel, Thallium, and Vanadium

Whole blood, dried blood spot on filter paper card

DCPAH

Toxic element panel, biopsy

Copper and iron toxicity

Liver

DCPAH

Trichothecenedon

 

 500 g feed

UCD;

Tissue macro/micro elemental analysis

arsenic, barium, cadmium, calcium, chromium, cobalt, copper, iron, lead, magnesium, manganese, molybdenum, phosphorus, potassium, sodium, sulfur, zinc

1 g Liver, kidney

WSU

Trace element screen

Copper, zinc, iron (unbound), magnesium, calcium, sodium, potassium, phosphorous

1-2 mL serum, 1-2 mL plasma (EDTA/heparin).

NDSU; UCD;

calcium, copper, iron, magnesium, phosphorus, zinc - sodium on request

1 mL separated plasma

WSU

Urea

Chill/freeze

WSU- 50 g feed, source, GI contents; TVMDL- 500 g feed, 20 mL water

TVMDL; WSU

Vitamin A

Avoid preservatives such as Citrate or EDTA. No Gel Clot Vacutainer Tubes.

10 g liver, 5 mL separated serum/plasma, 25 g feed.

CO; UCD

1 mL serum separated from clot. 

COR

Vitamin E

Separate serum and remove from clot immediately. Protect from light and keep cool.

10 g liver, 5 mL separated serum/plasma, 10 g feed.

CO; TVMDL; UCD

1 mL serum separated from clot. Do not use SST tubes

COR; WSU

Contact lab

PUR

Vomitoxin (DON) panel

DON (vomitoxin); 15-ADON; 3-ADON; Nivalenol; Zearalenone

 

At least 100 grams of sample (quart size bag of grain,

 gallon size bag of silage, forage, hay)

NDSU

Vomitoxin (Deoxynivalenol)

 

100 g feed

COR

Contact lab

PUR

White Snakeroot (tremetol)

 

Contact lab

PUR

Zearalenone

 

100g feed (COR),  500 g feed (UCD)

UCD; COR

Contact lab

PUR